Robust Multi-Read Reconstruction from Contaminated Clusters Using Deep Neural Network for DNA Storage

DNA has immense potential as an emerging data storage medium. The principle of DNA storage is the conversion and flow of digital information between binary code stream, quaternary base, and actual DNA fragments. This process will inevitably introduce errors, posing challenges to accurate data recovery. Sequence reconstruction consists of inferring the DNA reference from a cluster of erroneous copies. A common assumption in existing methods is that all the strands within a cluster are noisy copies originating from the same reference, thereby contributing equally to the reconstruction. However, this is not always valid considering the existence of contaminated sequences caused, for example, by DNA fragmentation and rearrangement during the DNA storage process.This paper proposed a robust multi-read reconstruction model using DNN, which is resilient to contaminated clusters with outlier sequences, as well as to noisy reads with IDS errors. The effectiveness and robustness of the method are validated on three next-generation sequencing datasets, where a series of comparative experiments are performed by simulating varying contamination levels that occurring during the process of DNA storage.